Thermo Scientific™ T4 Polynucleotide Kinase (10 U/µL), 500 Units

Thermo Scientific™ T4 Polynucleotide Kinase (10 U/µL), 500 Units

Thermo Scientific™ T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction). The reaction is reversible. In the presence of ADP, T4 Polynucleotide Kinase exhibits 5'-phosphatase activity and catalyzes the exchange of phosphate groups between 5'-P-oligo-polynucleotides and ATP (exchange reaction). The enzyme is also a 3'-phosphatase.

Features

• Active in Thermo Scientific restriction enzyme, RT, and T4 DNA ligase buffers