Thermo Scientific™ TMT10plex™ Isobaric Label Reagent Set, 8 x 0.2 mg
On demandThermo Scientific™ TMT10plex™ Isobaric Label Reagent Set, 8 x 0.2 mg
Amine-reactive Thermo Scientific TMT10plex™ Isobaric Mass Tag Labeling Reagents Sets enable multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS).
This Tandem Mass Tag™ (TMT™) 10-plex reagent set contains ten different isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm and a mass reporter. The reagent set enables up to ten different peptide samples prepared from cells or tissues to be labeled in parallel and then combined for analysis. For each sample, a unique reporter mass (i.e., TMT10 126-131Da) in the low-mass region of the high-resolution MS/MS spectrum is used to measure relative protein expression levels during peptide fragmentation and tandem mass spectrometry.
For use with: Mass Spectrometer
Features
- Powerful – concurrent MS analysis of multiple samples increases sample throughput and enables relative quantitation of up to ten different samples derived from cells, tissues or biological fluids
- Consistent – identical reagent structure and performance among TMTzero™, TMTduplex™, TMTsixplex™ and TMT10plex™ reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and datasets
- Robust – increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
- Efficient – amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
- Compatible – optimized for use with high resolution Thermo Scientific MS/MS platforms, such as the Q Exactive, Orbitrap Elite™ and Orbitrap Fusion™ Tribrid™ instruments with data analysis fully supported by Proteome Discoverer™ 1.4
Contents
- TMT10–126 Label Reagent: 8 x 0.2 mg
- TMT10–127N Label Reagent: 8 x 0.2 mg
- TMT10–127C Label Reagent: 8 x 0.2 mg
- TMT10–128N Label Reagent: 8 x 0.2 mg
- TMT10–128C Label Reagent: 8 x 0.2 mg
- TMT10–129N Label Reagent: 8 x 0.2 mg
- TMT10–129C Label Reagent: 8 x 0.2 mg
- TMT10–130N Label Reagent: 8 x 0.2 mg
- TMT10–130C Label Reagent: 8 x 0.2 mg
- TMT10–131 Label Reagent: 8 x 0.2 mg
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