Thermo Scientific™ TMT10plex™ Isobaric Mass Tagging Kit

Thermo Scientific™ TMT10plex™ Isobaric Mass Tagging Kit

The Amine-reactive Thermo Scientific TMT10plex™ Isobaric Mass Tag Labeling Kit contains the labeling reagents, as well as associated reaction reagents, for multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS).

This Tandem Mass Tag™ (TMT™) 10-plex reagent set contains ten different isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm and a mass reporter. The reagent set enables up to ten different peptide samples prepared from cells or tissues to be labeled in parallel and then combined for analysis. For each sample, a unique reporter mass (i.e., TMT10 126-131Da) in the low-mass region of the high-resolution MS/MS spectrum is used to measure relative protein expression levels during peptide fragmentation and tandem mass spectrometry.

For use with: Mass Spectrometer

Features

• Powerful – concurrent MS analysis of multiple samples increases sample throughput and enables relative quantitation of up to ten different samples derived from cells, tissues or biological fluids
• Consistent – identical reagent structure and performance among TMTzero™, TMTduplex™, TMTsixplex™ and TMT10plex™ reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and datasets
• Robust – increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
• Efficient – amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
• Compatible – optimized for use with high resolution Thermo Scientific MS/MS platforms, such as the Q Exactive, Orbitrap Elite™ and Orbitrap Fusion™ Tribrid™ instruments with data analysis fully supported by Proteome Discoverer™ 1.4

Contents

• TMT10-126 Label Reagent, 3 x 0.8 mg
• TMT10-127N Label Reagent, 3 x 0.8 mg
• TMT10-127C Label Reagent, 3 x 0.8 mg
• TMT10-128N Label Reagent, 3 x 0.8 mg
• TMT10-128C Label Reagent, 3 x 0.8 mg
• TMT10-129N Label Reagent, 3 x 0.8 mg
• TMT10-129C Label Reagent, 3 x 0.8 mg
• TMT10-130N Label Reagent, 3 x 0.8 mg
• TMT10-130C Label Reagent, 3 x 0.8 mg
• TMT10-131 Label Reagent, 3 x 0.8 mg
• Dissolution Buffer (1M triethyl ammonium bicarbonate), 5 mL
• Denaturing Reagent (10% SDS), 1 mL
• Reducing Reagent (0.5M TCEP), 1 mL
• Iodoacetamide, 12 × 9 mg
• Quenching Reagent (50% hydroxylamine), 1 mL
• Pierce Trypsin Protease, MS Grade, 5 × 20 µg
• Trypsin Storage Solution, 250 µL
• Albumin, Bovine, 2.5 mg