Thermo Scientific™ TMTduplex™ Isobaric Mass Tagging Kit

Thermo Scientific™ TMTduplex™ Isobaric Mass Tagging Kit

The Amine-reactive Thermo Scientific TMT™ Isobaric Mass Tagging Kit contains labeling reagents and associated reaction reagents that enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry.

Tandem Mass Tag™ 2-plex (TMTduplex™) Reagents are sets of isobaric compounds (i.e., same mass and structure, also called isotopomers) that are NHS-activated for covalent, irreversible labeling of primary amines (—NH2) groups. Each isobaric reagent contains a different number of heavy isotopes in the mass reporter region, which results in a unique reporter mass during tandem MS/MS for sample identification and relative quantitation. The reagents label all peptides prepared from cell or tissue samples for analysis of two samples in a single MS analysis.

For use with equipment: Mass Spectrometer

Features

• Powerful—concurrent MS analysis of two samples increases throughput and enables relative quantitation of two different treatment conditions
• Consistent—identical reagent structure and performance among TMTzero™, TMTduplex™, TMTsixplex™ and TMT10plex™ reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and data sets
• Robust—consistent chemistry allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research
• Efficient—amine-reactive NHS-ester activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
• Compatible—optimized for use with high resolution Thermo Scientific MS/MS platforms, such as the Q Exactive, Orbitrap Elite™ and Orbitrap Fusion™ Tribrid™ instruments with data analysis fully supported by Proteome Discoverer™ 1.0 and above

Contents

• TMT0 Label Reagent, 5 x 0.8 mg
• TMT2-126 Label Reagent, 5 x 0.8 mg
• TMT2-127 Label Reagent, 5 x 0.8 mg
• Dissolution Buffer (1M triethyl ammonium bicarbonate), 5 mL
• Denaturing Reagent (10% SDS), 1 mL
• Reducing Reagent (0.5M TCEP), 1 mL
• Iodoacetamide, 12 x 9 mg
• Quenching Reagent (50% hydroxylamine), 1 mL
• Trypsin, 2 x 20 µg
• Trypsin Storage Solution, 250 µL
• Albumin, Bovine, 2.5 mg