Thermo Scientific™ TMTsixplex™ Isobaric Mass Tagging Kit, 35 Reactions

Thermo Scientific™ TMTsixplex™ Isobaric Mass Tagging Kit, 35 Reactions

Amine-reactive Thermo Scientific TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry.

Tandem Mass Tag™ 6-plex (TMTsixplex™) Reagents are sets of isobaric compounds (i.e., same mass and structure, also called isotopomers) that are NHS-activated for covalent, irreversible labeling of primary amines (–NH2) groups. Each isobaric reagent contains a different number of heavy isotopes in the mass reporter region, which results in a unique reporter mass during tandem MS/MS for sample identification and relative quantitation. The reagents label all peptides prepared from cell or tissue samples for analysis of up to six samples in a single MS analysis.

For use with: Mass Spectrometer

Features

• Powerful – concurrent MS analysis of multiple samples increases sample throughput and enables relative quantitation of up to six different samples derived from cells, tissues or biological fluids
• Consistent – identical reagent structure and performance among TMTzero™, TMTduplex™, TMTsixplex™ and TMT10plex™ reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and datasets
• Robust – consistent chemistry allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research
• Efficient – amine-reactive NHS-ester activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
• Compatible – optimized for use with high resolution Thermo Scientific MS/MS platforms, such as the Q Exactive, Orbitrap Elite™ and Orbitrap Fusion™ Tribrid™ instruments with data analysis fully supported by Proteome Discoverer™ 1.0 and above

Contents

• TMT0 Label Reagent, 5 x 0.8 mg
• TMT6-126 Label Reagent, 5 x 0.8 mg
• TMT6-127 Label Reagent, 5 x 0.8 mg
• TMT6-128 Label Reagent, 5 x 0.8 mg
• TMT6-129 Label Reagent, 5 x 0.8 mg
• TMT6-130 Label Reagent, 5 x 0.8 mg
• TMT6-131 Label Reagent, 5 x 0.8 mg
• Dissolution Buffer (1M triethyl ammonium bicarbonate), 5 mL
• Denaturing Reagent (10% SDS), 1 mL
• Reducing Reagent (0.5 M TCEP), 1 mL
• Iodoacetamide, 12 x 9 mg
• Quenching Reagent (50% hydroxylamine), 1 mL
• Trypsin, 5 x 20 µg
• Trypsin Storage Solution, 250 µL
• Albumin, Bovine, 2.5 mg