Thermo Scientific™ Verso 1-step RT-qPCR Mix, ROX, 200 Reactions

Thermo Scientific™ Verso 1-step RT-qPCR Mix, ROX, 200 Reactions

The Thermo Scientific Verso 1-step RT-qPCR ROX Mix Kit for probe chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

For use with: 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
 

Features

• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube—convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR—improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction set up

Contents

• Verso Enzyme Mix: 1 x 50 µL
• RT enhancer: 1 x 250 µL
• 2X 1-Step qPCR ROX Mix: 2 x 1.25 mL
• Sufficient for 200 x 25 µL reactions.