Thermo Scientific™ Ion 16S™ Metagenomics Kit

Thermo Scientific™ Ion 16S™ Metagenomics Kit

The Ion 16S™ Metagenomics Kit is designed for rapid, comprehensive and broad-range analyses of mixed microbial populations using the Ion Torrent™ semiconducter sequencing workflow. The kit permits PCR amplification of hypervariable regions of the 16S rDNA gene from bacteria. The amplified fragments can then be sequenced using the Ion PGM™ Sequencing 400 Kit on the Ion PGM™ platform and analyzed using the Ion 16S™ metagenomics analyses module within the Ion Reporter™ software.

Features

• Comprehensive: The kit includes 2 sets of primers that can be used to amplify the corresponding hypervariable regions of the 16S rDNA gene in bacteria
• Flexible: The primer sets are provided as two pools. Each pool can be used individually or in combination to allow for flexibility in experimental set up
• Complete Workflow: Sequences generated using the Ion 16S™ Metagenomics Kit can be directly analyzed using the Ion 16S™ metagenomics analyses module within the Ion Reporter™ software enabling a rapid and semi-quantitative assessment of complex microbial samples

Contents

• DNA dilution buffer (7 mL)
• 2 tubes 2X environmental master mix (0.8 mL per tube)
• E. coli DNA control (40 µL)
• Negative control water (1 mL)
• 16S primer set V2-4-8 (300 µL)
• 16S primer set V3-6,7-9 (300 µL)