Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase, 20
On demandThermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase, 20
Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription. For reverse transcription the kit uses Thermo Scientific Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.
Features
- Integrated genomic DNA removal step
- Increased reaction temperatures—the first strand of cDNA can be synthesized within the 42 to 65°C temperature range
- High yields of full-length first strand cDNA—with RNA templates up to 20 kb
- Flexible priming—oligo(dT)18, random hexamer, or gene-specific primers
Contents
- Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase contains Maxima H Minus Enzyme Mix, dsDNase, 10X dsDNase Buffer, Oligo(dT)18 and Random hexamer primers, 5X RT Buffer, dNTP Mix, and nuclease-free water.
There are no report
You May Also Like
![](https://laboshop.ae/public/storage/files/5bd49deb-c4f6-47bf-98d7-d38c5d2ed641.jpg)
Applied Biosystems™ QuantStudio™ 3/5 10-Dye Spectral Calibration Kit, 96-well, 0.2 mL
$ 1,430 $ 1,573
In stock
![](https://laboshop.ae/public/storage/products/August2022/ZMhnY12ht7NmL9qbWa78.jpg)
Applied Biosystems™ ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well
$ 62
In stock
![](https://laboshop.ae/public/storage/files/c6c6aaec-cd66-4bf5-9353-6eee6ecd9278.jpg)
Applied Biosystems™ Megaplex™ Primer Pools, Human Pools Set v3.0
$ On demand
On demand
![](https://laboshop.ae/public/storage/products/September2022/4StqRJd24VXE2gi4Qudt.jpg)
INOVIA™ INOGene-SCoV-2 RT-qPCR (Covid-19) Detection Kit, 100 reactions
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/G5BMyPfRCOSALtB61wdTecafj6HKBt6PG38hh6aO.jpg)
Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG, 5 mL
$ On demand