Thermo Scientific™ Maxima Reverse Transcriptase (200 U/µL), 2000
On demandThermo Scientific™ Maxima Reverse Transcriptase (200 U/µL), 2000
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity. The engineered enzyme features dramatically improved thermostability and robustness, and increased synthesis rates compared to wild type M-MuLV RT. Maxima Reverse Transcriptase is capable of reproducible cDNA synthesis from a wide range of input total RNA amounts (from 1 pg to 5 µg) at elevated temperatures (50 to 65°C), which makes this enzyme an ideal tool for two step RT-qPCR (see supporting data). Due to its high thermostability, Maxima enzyme maintains full activity during the entire reverse transcription reaction, generates the highest yields of cDNA, and is able to synthesize even very long RNA transcripts up to 20 kb. The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. Due to its increased synthesis rate, the reverse transcriptase reaction can be completed in 15 to 30 min.
Features
- High yields of full-length cDNA up to 20 kb
- Active up to 65°C
- Thermostable—90% active after incubation at 50°C for 60 minutes
- High sensitivity—reproducible cDNA synthesis from a wide range of total RNA quantities (1 pg to 5 µg)
- Efficient—complete cDNA synthesis in 15 to 30 minutes
- Incorporates modified nucleotides
Contents
- Maxima Reverse Transcriptase (provided as 2000 Units at 200 U/µL concentration) is supplied with 5X RT Buffer (250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT).
There are no report
You May Also Like
Applied Biosystems™ QuantStudio™ 3/5 10-Dye Spectral Calibration Kit, 96-well, 0.2 mL
$ 1,430 $ 1,573
In stock
Applied Biosystems™ ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well
$ 62
In stock
Invitrogen™ SuperScript™ IV Reverse Transcriptase, 40000 Units
$ On demand
On demand
Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, 250 Reactions
$ On demand