Thermo Scientific™ MuSeek Library Preparation Kit, Illumina compatible
On demandThermo Scientific™ MuSeek Library Preparation Kit, Illumina compatible
Thermo Scientific™ MuSeek™ Library Preparation Kit, Illumina™ compatible is designed for generation of high‐quality genomic DNA libraries for sequencing with the Illumina™ MiSeq™ and HiSeq™ systems. The fast protocol utilizes the MuA transposase enzyme, which catalyzes simultaneous fragmentation of double-stranded target DNA and tagging the fragment ends with transposon DNA. In a subsequent PCR step the platform-specific adapters are added using a robust and accurate Thermo Scientific Phusion™ High-Fidelity DNA polymerase. Starting with only 50 ng or 100 ng of sample the protocol can be used to generate 100 bp - 1,000 bp insert libraries. The kit contains components sufficient for 12 fragmentation reactions and the subsequent adapter-addition polymerase chain reactions. Sequencing primers required for sequencing libraries prepared by this method are provided as well.
For use with equipment: Illumina™ HiSeq 2500, Illumina ™ MiSeq.
Features
- Convenient—no physical shearing, end-repair and ligation steps
- Fast—generates NGS fragment libraries in less than 90 minutes
- Low sample input—DNA fragment libraries from 50 ng DNA input
- Indexing compatible—MuSeek Index Set 1 enables preparation of up to 24 dual-indexed DNA libraries for sequencing in one run.
Contents
- MuSeek Enzyme Mix, IL
- MuSeek Fragmentation Reaction Buffer
- MuSeek Stop Solution
- Water, nuclease-free
- Control DNA
- MuSeek Adapter Addition Primer Mix, IL
- MuSeek Adapter Addition Reaction Buffer, IL
- Phusion™ Hot Start II High-Fidelity DNA Polymerase, IL
- MuSeek Sequencing Primer, Read 1
- MuSeek Sequencing Primer, Read 2
- MuSeek Sequencing Primer, Index Read
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