Thermo Scientific™ North2South™ Biotin Random Prime DNA Labeling Kit

Thermo Scientific™ North2South™ Biotin Random Prime DNA Labeling Kit

Thermo Scientific North2South Random Prime DNA Biotinylation Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridization and detection methods.

Features

• Works with as little as 100ng starting DNA template
• Exonuclease-free Klenow fragment in the DNA labeling kit produces higher yields
• Each reaction yields probe sufficient for approximately three (10 x 10 cm) blots

Contents

• Heptanucleotide Mix (5X): 100 µL
• Deoxynucleotide Mix (5X): 80 µL
• Reaction Buffer (10X): 50 µL
• DNA Polymerase Klenow Fragment (5U/µL): 10 µL
• Control DNA (250 ng/µL): 5 µL
• Ammonium Acetate (5M): 1 mL
• EDTA (0.5M, pH 8.0): 1 mL
• Nuclease-free Water: 1 mL
• Biotin-11-dUTP: 20 µL