Thermo Scientific™ North2South™ Chemiluminescent Hybridization and Detection Kit

Thermo Scientific™ North2South™ Chemiluminescent Hybridization and Detection Kit

Thermo Scientific North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.

Features

• Sensitivity equal to or greater than 32P
• Much faster than DIG detection systems
• Amenable to nearly any DNA or RNA hybridization method
• Optimized hybridization and blocking buffers and protocol steps yield consistent results from experiment to experiment
• High level of light output and consistent low background allows for very short and multiple exposure times using cooled CCD camera systems or film
• Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
• Specific plaques can be picked out of areas that are very dense with signal due to extremely low background
  

Contents 

• Stabilized Streptavidin-HRP: 1.5 mL
• Luminol/Enhancer Solution: 80 mL
• Stable Peroxide Solution: 80 mL
• Hybridization Buffer: 125 mL
• Hybridization Stringency Wash Buffer (2X): 375 mL
• Blocking Buffer: 500 mL
• Wash Buffer (4X): 500 mL
• Substrate Equilibration Buffer: 500 mL