Applied Biosystems™ TaqMan™ Advanced miRNA Human A and B Cards

Applied Biosystems™ TaqMan™ Advanced miRNA Human A and B Cards

The Applied Biosystems TaqMan Advanced miRNA Human A and B Cards provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 384-well microfluidic card format. This product contains two cards, enabling accurate quantitation of 754 unique miRNAs in as little as five hours. Four TaqMan Advanced miRNA Assay endogenous controls are included in each card to aid in data normalization. Details about the included assays are available in the Target List file. The assays can detect and quantify the mature form of the miRNA from 1–10 ng of total RNA from tissue.

The kit enables the analysis of:

• Multiple miRNAs from a single amplified sample

• Samples that are limited in quantity, including serum, plasma, or other biological fluids

Features

• Universal reverse transcription (RT)—one RT step for all TaqMan Advanced miRNA assays
• Sensitive—detect as few as 60 copies of input miRNA
• Specific—detect only mature miRNA and distinguish between highly homologous miRNAs
• Small sample input—detect and quantify mature miRNA from as little as 1–10 ng of total RNA or 2 μL of plasma or serum
• Versatile—compatible with tissue and biofluids, including serum and plasma

Contents

• One 384-well array