Applied Biosystems™ MethylMiner™ Methylated DNA Enrichment Kit

Applied Biosystems™ MethylMiner™ Methylated DNA Enrichment Kit

The MethylMiner™ Methylated DNA Enrichment Kit is for highly sensitive enrichment of methylated DNA for downstream analysis including PCR/qPCR based assays, bisuflite conversion followed by amplification, cloning, and sequencing, direct sequencing, library preparation for high-throughput sequencing, and sample- prep for DNA microarray analysis. Patterns of DNA methylation seem to be important for making determinations about development and diseases such as cancer. This kit enables superior enrichment and differential fractionation of double-stranded DNA based on CpG methylation density, with increased sensitivity over antibody-based methods. Fractionation permits important comparisons between samples and enables researchers to focus analysis on only the methylation densities of interest.

Features

• Partitioning with high affinity binding—at least 4-fold greater sensitivity than antibody-based methods
• Fractionation based on CpG methylation density—ds DNA capture is achieved with MBD2 protein and facilitates ligation of double-stranded adapters for next-generation sequencing
• Rapid and easy elution with salt eliminates the need for proteinase K treatment and phenol:chloroform extraction
• Precise answers—fractionated DNA permits distinctions to be made regarding methylation status and density
• Fast protocol—completed in less than 4 hours
• Easy—simple handling with Dynabeads® the gold standard magnetic beads

Contents

• Dynabeads™ M-280 Streptavidin, Buffers for binding, washing, and elution which need to be stored at 4° C. The kit also includes methylated and non-methylated DNA, blocking DNA, glycogen, and controls primers which need to be stored at -20°C. The MBD-biotin protein provided in the kit should be stored at -80° C.