Applied Biosystems™ Precision ID mtDNA Control Region Panel

Applied Biosystems™ Precision ID mtDNA Control Region Panel

The Applied Biosystems Precision ID mtDNA Control Region Panel is an innovative next-generation sequencing (NGS) approach to mitochondrial DNA (mtDNA) analysis specifically developed for forensic applications. It is a 2-pool multiplex assay that targets the 1.2 kb control region of the human mitochondrial genome, which encompasses HV-I,II, and III (see figure below). This panel was constructed using a novel, innovative tiling approach to obtain optimal coverage data from highly compromised, degraded samples such as hair shafts, teeth, and bones that uses massively parallel sequencing built on Ion AmpliSeq technology. This mtDNA tiling approach was also used to construct the Precision ID mtDNA Whole Genome Panel.

For use with equipment: Ion GeneStudio S5 System, Ion PGM™ System, Ion S5™ XL System, Ion OneTouch™ 2 System, Ion Chef™ System.

Features

• Small amplicons enable control region coverage of degraded samples
• As little as 2 pg of input
• Automated library and template preparation using the Ion Chef System
• Optimized analysis using Converge software
• Multiple chip formats to meet a range of sample throughput requirements
• Ability to multiplex up to 56 samples
• Less than 45 minutes of hands-on time for a DNA-to-data targeted sequencing workflow
• Total sample-to-data time of less than 2 days

Contents

• 2 tubes (Pool 1 and Pool 2) Precision ID mtDNA Control Region Panel