MGIEasy Exome Capture V4 Probe Set（16 RXN）

MGIEasy Exome Capture V4 Probe Set（16 RXN）

MGIEasy Exome Capture V4 Probe Set is the first exome capture product that is independently designed by MGI. The databases used for probe design include CCDS, GENCODE, RefSeq, miRBase and so on. The probe set can be flexibly matched with MGIEasy Exome Universal Library Prep Set or MGIEasy Exome FS Library Prep Set for the construction and hybridization of libraries for MGI sequencing platforms; it can also be matched with other library preparation kits for other high throughput sequencing platforms.

Features

• Probe regions 59Mb, covering over 20000（refGene）genes: The databases used for probe design include CCDS, GENCODE, RefSeq, miRBase and so on.
• Deep and comprehensive coverage: In HGMD and ClinVar databases, MGIEasy Exome Capture V4 Probe can cover 97% and 85% of mutation sites respectively, playing an important role in disease research and diagnosis.
• Stable and efficient capture rate: The capture rate is over 75%.
• High consistency rate of variant detection: For cell line NA12878，the consistency rate of MGI Probe V4 is 99.2% and 87% for SNV and indels, respectively.
• Compatible with various library preparation methods and sequencing platforms: Compatible with MGIEasy Exome Universal Library Prep Kit and MGIEasy Exome FS Library Prep Kit, as well as other library preparation kits. Compatible with sequencing platforms from MGI, Illumina and Thermo Fisher.
• Probe lenght: 90nt.
• Covered regions: Exome regions, >99% disease-related regions.
• DNAinput: 1μg PCR product for one sample per hybridization, >250ng each sample for multiple samples per hybridizationand 1μg ≤total input≤2μg.
• Number of samples per hybridization: 1-8.
• Technologies: Capture+NGS.

Content

• Probes, MGIEasy Exome Capture V4 Probe（16RXN): 1000007740
• Hybridization and elution, MGIEasy MGIEasy Exome Capture Elution Kit（16RXN): 1000007742