Ion Torrent™ Oncomine™ Myeloid Research Assay- Chef Ready
On demandIon Torrent™ Oncomine™ Myeloid Research Assay- Chef Ready
The Ion Torrent Oncomine Myeloid Research Assay consists of three pools of AmpliSeq oligonucleotide primers and associated reagents to generate amplicon libraries for next-generation sequencing (NGS) on Ion Torrent platforms. The assay is designed to provide sensitive and comprehensive sample amplification of relevant DNA mutations and fusion transcripts associated with myeloid disorders in a single NGS run. The panel is comprised of 40 key DNA genes and a broad fusion panel of 29 driver genes to cover the most relevant targets in major myeloid disorders: acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML).
Features
- Simple, robust workflow that encompasses mutations and fusions in two DNA and one RNA pool in a single NGS run
- Excellent coverage of challenging targets such as CEBPA and FLT3-ITDs
- Multiplex up to four samples on a single Ion 318 chip or twelve on an Ion 530 chip
- Compatible with as little as 10 ng/pool input DNA or RNA per library from blood or bone marrow samples
- Validated detection of somatic variants down to 5% allele frequency Simple and fast workflow produces targeted libraries in 3.5 hours typically
- For use on the Ion PGM and Ion S5 systems with manual or automated library preparation
- Compatible with the Oncomine Knowledgebase Reporter for variant annotation
Contents
- 2X Oncomine™ Myeloid DNA Panel (pool 1 of 2)
- 2X Oncomine™ Myeloid DNA Panel (pool 2 of 2)
- 2X Oncomine™ Myeloid DNA Panel (pool 2 of 2)
- Ion AmpliSeq™ Chef Reagents DL8
- Ion AmpliSeq™ Chef Supplies DL8 (per insert): Ion AmpliSeq™ Tip Cartridge L8 - PCR Frame Seal - Enrichment Cartridge
- IonCode™ 0101–0132 in 96 Well PCR Plates (dried)
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