Applied Biosystems™ PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean with Proteinase K

Applied Biosystems™ PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean with Proteinase K

The PrepSEQ® Spin Sample Preparation Kit – Extra Clean with Proteinase K is recommended for difficult to lyse bacteria, like Listeria monocytogenes, that also has a high lipid content, such as soft cheeses. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications.

Features

• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol
• For Use With (Equipment): Centrifuge
• Sample Type: Any Swab Type, Bacteria (Gram-Negative), Bacteria (Gram-Positive), Bacterial Culture, Food (High Lipid Content), Water Samples, Bacteria (Hard-to-Lyse)
• Final Product Type: Bacterial DNA
• Starting Material Amount: 750 µL

Contents

• Spin columns: 100
• Microcentrifuge tubes 1.5 mL: 2 × 100
• Lysis Buffer: 1 bottle 5 mL
• Proteinase K (20 mg/mL): 1 tube 1.25 mL