Chromo Pfu DNA Polymerase (Pyrococcus furiosus), 500u

Chromo Pfu DNA Polymerase (Pyrococcus furiosus), 500u

Chromo Pfu DNA Polymerase is an extremely thermostable proofreading DNA polymerase, suitable for applications requiring high temperatures synthesis of DNA. Pfu DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg2+. It exhibits the 3' to 5' proofreading activity. The enzyme is supplemented with inert color tracer dyes.

Features

• Color tracer dyes for ease of visualization of the addition of polymerase to the reaction.
• Use of 1u in every 25μl final reaction allows direct loading and serves as tracking dyes during gel electrophoresis. The blue and pink color dyes migrate approximately at 4kb and 0.3kb respectively on 1% TAE agarose gel.
• Ultra pure recombinant protein allows amplification up to 8kb
• 5X ViBuffer Pfu provided for both short and long amplification.
• Recommended for use in high-fidelity amplification and cloning of blunt-ended amplification products.

Contents

• Chromo Pfu DNA Polymerase (Pyrococcus furiosus), 500u.
• 10X ViBuffer A (without MgCl2).
• 10X ViBuffer S.
• 50mM MgCl2.