CleanPlex® Hereditary Cancer Panel v2, 96rxn

CleanPlex® Hereditary Cancer Panel v2, 96rxn

The CleanPlex® Hereditary Cancer Panel v2 is a targeted resequencing assay designed for analyzing genes associated with an increased risk of developing hereditary cancers on Illumina and Ion Torrent™ sequencing platforms. Associated cancer includes breast cancer, ovarian cancer, uterine cancer, skin cancer, prostate cancer, gastric cancer, colorectal cancer, pancreatic cancer, etc. The panel is expertly curated using the latest research findings to target 37 genes and both single nucleotide variants (SNVs) and insertion-deletion mutations (indels). Furthermore, it also detects hotspot mutations rs12516 and rs8176318 in the BRCA1 3’ UTR and structural rearrangement of exons 1-7 in MSH2 (Boland inversion)*. This panel is specifically designed to detect inherited mutations and is not appropriate for the detection of other types of mutations in acquired cancers. Starting with 20 ng of high-quality genomic DNA (10 ng per primer pool), sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours.

Contents

The CleanPlex Hereditary Cancer Panel v2 contains CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex® Dual-Indexed PCR Primers Set A 96rxn OR CleanPlex® Single-Indexed PCR Primers for Ion Torrent™, CleanMag®Magnetic Beads and CleanMag® Magnetic Rack or CleanMag® Magnetic Plate are ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.