Gibco™ Bac-to-Bac™ Baculovirus Expression System

Gibco™ Bac-to-Bac™ Baculovirus Expression System

The Bac-to-Bac baculovirus expression system enables the efficient production of recombinant baculovirus for expression testing in insect cells. The system relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells.

Features

• Time-saving expression bacmid
• Easy colony screening—The parent bacmid in DH10Bac E. coli contains a segment of the lacZa gene. 
• High transfection efficiency with ExpiFectamine Sf Transfection Reagent
• Flexibility—The Bac-to-Bac system comes with a pFastBac vector that contains a large multiple cloning site (MCS) for simplified cloning. 
• High protein expression—The pFastBac vector uses the strong polyhedrin promoter to generate high levels of expression in a variety of insect cell line such as Sf9, Sf21, and High Five cells

Contents

• pFastBac1 vector (10 µg)
• pFastBac 1-Gus control vector (4 ng)
• MAX Efficiency DH10Bac Chemically Competent E. coli (1 kit with 5 x 0.1 mL)
• ExpiFectamine Sf Transfection Reagent (1 mL)