Gibco™ Bac-to-Bac™ C-His TOPO™ Expression System

Gibco™ Bac-to-Bac™ C-His TOPO™ Expression System

Bac-to-Bac baculovirus expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells. 

Features

• Flexibility—The Bac-to-Bac C-His TOPO vector contains a C-terminal His-tag with a TEV cleavage site for easy purification of His- tagged proteins with nickel-chelating resins (such as the Invitrogen ProBond Purification System) and generation of native proteins with the aid of the Invitrogen AcTEV Protease
• Fast cloning, easy screening—Blunt TOPO cloning technology enables cloning of blunt-end PCR products into the pFastBac TOPO vector in five minutes
• High transfection efficiency with ExpiFectamine Sf Transfection Reagent
• Time-saving expression bacmid
• Easy colony screening—The parent bacmid in DH10Bac E. coli contains a segment of the lacZa gene

Contents

• Vector kit for 20 TOPO cloning reactions

   –pFastBac/CT-TOPO vector containing the C-terminal TEV cleavage sit and His-tag

   –pFastBac 1-Gus control plasmid (control expression vector)

   –Other kit reagents: 10X PCR, dNTP mix, Salt solution, sterile water, control PCR template, polyhedrin forward primer, SV40 pA reverse primer

• One Shot Mach1-T1R Chemically Competent E. coli for 20 reactions: 20 x 50 µL
• MAX Efficiency DH10Bac Competent E. coli for 20 reactions: 4 kits with 5 x 0.1 mL each
• ExpiFectamine Sf Transfection Reagent: 1 mL