Gibco™ Bac-to-Bac™ C-His TOPO™ Cloning Kit

Gibco™ Bac-to-Bac™ C-His TOPO™ Cloning Kit

Bac-to-Bac® Baculovirus Expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells

Features

• Reliable and fast protein expression
• Traditional baculovirus systems require purification and amplification of an initial low-titer viral supernatant. 
• High expression, easy screening

Contents

•  Vector kit for 20 topo cloning reactions:

      - pFastBac⁄CT-TOPO™ Vector containing the C-terminal TEV cleavage sit and His-Tag.

      - pFastBac⁄CT-Gus Control Plasmid (Control expression vector)

      - Other reagents supplied: 10x PCR, dNTP Mix, Salt Solution, Sterile Water, Control PCR template, Polyhedrin Forward Primer, SV40 pA Reverse Primer

• Kit containing competent cells (20 reactions):

      - One Shot™ Mach1-T1R Chemically Competent E. coli

• Vector kit
• One Shot™ Mach1-T1R Chemically Competent E. coli