Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells, 50 Reactions

Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 50 Reactions
Order number : K280040
($) On demand

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells, 50 Reactions


Zero Blunt® TOPO® PCR Cloning Kit provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Each kit has the Zero Blunt® TOPO® vector (see figure) containing the ccdB gene for positive selection, only permitting growth of plasmid vectors with recombinants. Zero® Blunt® TOPO® kits are available with a variety of competent cells, or without competent cells, depending on your needs and budget.


Features

  • Fast and easy—go from PCR to clones in just 3 steps and in as little as 5 minutes hands-on time
  • Efficient—obtain up to 95% clones with correct insert
  • Proven—reliable performance for over a decade with over 4,000 citations
  • Simple —no ligase, post-PCR procedures, or PCR primers containing specific sequences are required.

 

Contents

Box 1:

  • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
  • Salt solution
  • dNTPs
  • Control template
  • M13 forward and reverse primers
  • Sterile water

Box 2:

  • One Shot™ Chemically Competent or Electrocomp™ E. coli
  • S.O.C. medium
  • Supercoiled pUC19 control plasmid
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