Gibco™ Bac-to-Bac™ N-His TOPO™ Cloning Kit
On demand
Order number :
A11099
Gibco™ Bac-to-Bac™ N-His TOPO™ Cloning Kit
Bac-to-Bac® Baculovirus Expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells.
Features
- Reliable and fast protein expression
- Traditional baculovirus systems require purification and amplification of an initial low-titer viral supernatant.
- High expression, easy screening
Contents
- Vector kit for 20 topo cloning reactions:
- pFastBac⁄NT-TOPO™ Vector containing the N-terminal TEV cleavage sit and His-Tag.
- pFastBac⁄NT-Gus Control Plasmid (Control expression vector)
- Other reagents supplied: 10x PCR, dNTP Mix, Salt Solution, Sterile Water, Control PCR template, Polyhedrin Forward Primer, SV40 pA Reverse Primer
- Kit containing competent cells (20 reactions):
- One Shot™ Mach1-T1R Chemically Competent E. coli
- Vector kit
- One Shot™ Mach1-T1R Chemically Competent E. coli
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