Ion Torrent™ Oncomine™ Comprehensive Assay Plus, Manual library preparation

Ion Torrent™ Oncomine™ Comprehensive Assay Plus, Manual library preparation

The Oncomine Comprehensive Assay Plus is a targeted, next-generation sequencing (NGS) assay that provides a comprehensive genomic profiling solution appropriate for formalin-fixed paraffin-embedded (FFPE) tissues from solid tumors. The assay detects multiple biomarkers associated with targeted and immune checkpoint therapies. It allows concurrent analysis of DNA and RNA to simultaneously detect multiple types of variants in a single workflow, including hotspots, single nucleotide variants (SNVs), insertions and deletions (InDels), copy number variants (CNVs), microsatellite instability (MSI), and tumor mutational burden (TMB), automatically calculated tumor cellularity, loss-of-heterozygosity, and gene fusions featuring FusionSync.

Features

• Enables analysis of variants across 500+ genes
• Detection of SNVs, CNVs, InDels, TMB, MSI, and gene fusions
• Robust performance from as little as 10 ng per pool of DNA/RNA isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and controls
• Content driven by the Oncomine Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published evidence

Contents

• 2X DNA OCA Plus (pool 1 of 2)
• 2X DNA OCA Plus (pool 2 of 2)
• RMC 2X RNA OCA Plus (pool 1 of 2)
• 2X RNA OCA Plus (pool 2 of 2)
• 5X Ion AmpliSeq™ HiFi Mix (red cap)
• FuPa Reagent (brown cap)
• Switch Solution (yellow cap)
• DNA Ligase (blue cap)
• 25X Library Amp Primers (pink cap)
• 1X Library Amp Mix (black cap)
• Low TE