Ion Torrent™ Oncomine™ Comprehensive Assay Plus, RNA, Manual library preparation

Ion Torrent™ Oncomine™ Comprehensive Assay Plus RNA, Manual library preparation

The Oncomine Comprehensive Assay Plus RNA contains a targeted, multi-biomarker panel that enables highly sensitive and robust detection of known and novel fusions covering >1300 isoforms from 49 fusion drivers associated with solid tumors.

Features

• Detection of targeted known-fusion detection, non-targeted detection of novel fusions, and novel fusion detection with unknown partners
• Highly sensitive and robust methodology with the ability to detect tiny levels of a tumor-specific fusion transcript in background normal RNA
• Robust performance from as little as 10 ng per pool of RNA isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and controls
• Content driven by the Oncomine Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published evidence

Contents

• 2X RNA OCA Plus (pool 1 of 2)
• 2X RNA OCA Plus (pool 2 of 2)
• 5X Ion AmpliSeq™ HiFi Mix (red cap)
• FuPa Reagent (brown cap)
• Switch Solution (yellow cap)
• DNA Ligase (blue cap)
• 25X Library Amp Primers (pink cap)
• 1X Library Amp Mix (black cap)
• Low TE