Invitrogen 100 µg MTCO1 Monoclonal Antibody (1D6E1A8)
On demandInvitrogen 100 µg MTCO1 Monoclonal Antibody (1D6E1A8)
Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB.
For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal. Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio. In Western blot applications, this antibody detects a band at approximately 40 kDa (predicted MW: 57 kDa). In mouse liver lysate a specific band below 37 kDa was detected.
Recommended positive controls:
- ICC/IF - Pig retinal pigment epithelial cells, rat cerebellum primary cells
- IHC (P) - Human skeletal muscle and colon tissue, rat pancreas tissue, pig smooth muscle tissue
- WB - Mouse, human, bovine and rat heart mitochondria lysate
- Flow - HEK-293 cells
There are no specifications
There are no report
You May Also Like
Invitrogen CD40 Monoclonal Antibody (1C10), Unconjugated, 500 µg, eBioscience™
$ On demand
On demand
Invitrogen 25 µg CD45R (B220) Monoclonal Antibody (RA3-6B2), Super Bright 436, eBioscience™
$ On demand
On demand
Invitrogen CD11c Monoclonal Antibody (N418), Biotin, 50 µg, eBioscience™
$ On demand
On demand
Invitrogen 25 µg CD44 Monoclonal Antibody (IM7), NovaFluor Yellow 570, eBioscience™
$ On demand
On demand
Invitrogen Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, 100 Test, eBioscience™
$ On demand
On demand
Invitrogen CD80 (B7-1) Monoclonal Antibody (2D10.4), Brilliant Ultra Violet™ 737, 100 Tests, eBioscience™
$ 482
On demand
Invitrogen CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), PerCP-eFluor 710, 25 Tests, eBioscience™
$ On demand