Invitrogen 100 µg Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 645, eBioscience™
On demand
Order number :
64-4321-82
Invitrogen 100 µg Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 645, eBioscience™
The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin, This eBR2a antibody has been reported for use in flow cytometric analysis.
- Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
- When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
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