Invitrogen 100 µg Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright 645, eBioscience™
On demand
Order number :
64-4031-82
Invitrogen 100 µg Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright 645, eBioscience™
The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin, This rat IgG2b, kappa, isotype control has been reported for use as a control for immunofluorescent staining, flow cytometric analysis, immunohistochemistry and immunocytochemistry.
- When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
- Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser
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