Invitrogen™ Alexa Fluor™ 594 Antibody Labeling Kit

Invitrogen™ Alexa Fluor™ 594 Antibody Labeling Kit

Molecular Probes® Alexa Fluor® antibody labeling kits provide a convenient means to label small amounts of antibodies with Alexa Fluor® dyes (choice of 10 colors). This kit is optimized for labeling 100 μg of antibody per reaction with infrared fluorescent Alexa Fluor® 790. Small amounts of other proteins (>40 kDa) can also be labeled with this kit.

The Alexa Fluor® 790 Antibody Labeling Kits contains everything you need to perform five separate labeling reactions as well as to purify the resulting conjugates. The conjugates are ideal for multiple applications, including small animal in vivo imaging, flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, indirect FISH, and more.

For use with equipment: Fluorescent Imager

Features

• With an excitation and emission maximum of 590/617 nm, Alexa Fluor® 594 can be efficiently excited using a 594 nm Kr or He-Ne laser line and detected under standard Texas Red™ filters
• Labeled proteins typically ready to use typically in 90 min (∼15 min hands-on time)
• Useful for labeling 100 µg of protein
• Optimized for small-scale labeling of any protein >40 kDa
• Purified using convenient spin filters
• Stabilizing proteins must be removed from the sample before labeling
• Includes detailed instructions for determining degree of labeling (DOL)