Thermo Scientific™ DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))

Thermo Scientific™ DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))

Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

DTSSP is 3,3'-Dithiobis(sulfosuccinimidylpropionate), which contains an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker.

Features

• Reactive groups: sulfo-NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
• Spacer arm also easily cleaved with reducing SDS-PAGE sample loading
• Cleavable crosslinker allows separation of crosslinked products
• Water-soluble; compare with DSP
• Membrane-impermeable, allowing for cell surface labeling