Invitrogen™ Amino Allyl MessageAmp™ II-96 aRNA Amplification Kit

Invitrogen™ Amino Allyl MessageAmp™ II-96 aRNA Amplification Kit

The Ambion® Amino Allyl MessageAmp™ II-96 aRNA Amplification Kit includes reagents for high- throughput aRNA amplification with amimo allyl NTP incorporation. The kit includes sufficient reagents for 100 reactions; Cy™3 dye is not included.

Features

• Consistently higher yields with magnetic bead- compared to filter-based purification
• Highly reproducible aRNA yields and size (CV less than 10%)
• Flexible format allowing RNA amplification with a partial or full 96-well plate
• Simple integration into robotic platforms

Contents

• The Amino Allyl MessageAmp™ II-96 aRNA Amplification Kit contains T7 Oligo(dT) Primer, ArrayScript™ RT, RNase Inhibitor, 10X First-Strand Buffer, dNTP Mix, 10X Second-Strand Buffer, DNA Polymerase, RNase H, T7 Enzyme Mix, T7 10X Reaction Buffer, T7 UTP Solution, aaUTP Solution, ATP⁄CTP⁄GTP Mix, Coupling Buffer, DMSO, Hydroxylamine and Control RNA (1 mg⁄mL HeLa total RNA) which are all stored at –20°C. cDNAPure and RNA Binding Beads are stored at +4°C. cDNA Wash Buffer Concentrate, aRNA Wash Solution Concentrate, Bead Resuspension Solution, aRNA Elution Buffer, aRNA Binding Buffer Concentrate, U-Bottom Plate, PCR Plate, Reservoir, Nuclease-free Water, and detailed Instruction Manual are stored at room temperature.