Thermo Scientific™ DecaLabel DNA Labeling Kit

Thermo Scientific™ DecaLabel DNA Labeling Kit

Thermo Scientific DecaLabel DNA Labeling Kit is an advanced system for fast synthesis of radiolabeled DNA probes of high specific activity. It's based on the improved random-primed method developed by Feinberg and Vogelstein.
The primary improvement over traditional random-primer kits involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C. In addition, Klenow Fragment, exo- has been genetically engineered to remove exonuclease activity. The improved enzyme does not degrade the labeled probe during reaction and allows high labeling yields even from low template amounts. DNA fragments of any length can be uniformly labeled. Two labeling mixes are provided for flexibility in using either radiolabeled dATP or dCTP.

Features

• High specific radioactivity of probes—greater than 1 x 109 dpm/µg DNA
• Fast—5 minutes at 37°C
• High yields with Klenow Fragment, exo no degradation of a labeled probe during reaction
• Flexible—suitable for either radiolabeled dATP or dCTP
• Includes Label or Dye: No
• Labeling Method: Direct Labeling
• No. of Reactions: 30
• Product Type: DNA Labeling Kit
• Detection Method: Radioactive
• Final Product Type: DNA
• Format: Kit
• Labeling Target: Genomic DNA, PCR Products, DNA (General)
  

Contents

• Klenow Fragment, exo–, 5 u/μL 30 μL 
• Decanucleotide in 5X Reaction Buffer: 300 μL 
• Mix A (minus dATP): 90 μL 
• Mix C (minus dCTP): 90 μL 
• dNTP Mix: 120 μL 
• Control Template λ DNA/HindIII fragments (10 μg/mL): 50 μL 
• Water, nuclease-free: 2X1.25 mL