Invitrogen™ Anza™ Alkaline Phosphatase, 2000 Reactions
On demand
Order number :
IVGN2208
Invitrogen™ Anza™ Alkaline Phosphatase, 2000 Reactions
Invitrogen™ Anza™ Alkaline Phosphatase is intended for use in the removal of 3' and 5' phosphate groups that remain after Anza™ restriction enzyme digestion, such as for the dephosphorylation of vectors prior to insert ligation and removal of 5′ phosphates prior to end-labeling. Since the 5′ phosphate group is required for DNA ligation, treatment of vectors with Anza Alkaline Phosphatase prevents self-ligation and re-circularization, resulting in decreased vector background when cloning.
Features
- Validated for use with all Anza restriction enzymes
- Dephosphorylates in 15 minutes at 37°C
- Heat inactivated after 5 minutes at 80°C
- Fully active in Anza Buffer
- Dephosphorylates all types of DNA ends (blunt, 5'- and 3'-overhangs)
Contnents
- 2 mL Anza Alkaline Phosphatase
- 1 mL Anza Buffer (10X)
- 1 mL Anza Red Buffer (10X)
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