Invitrogen™ BacMam Histone H3K27me3 Cellular Assay Kit

Invitrogen™ BacMam Histone H3K27me3 Cellular Assay Kit

The BacMam Histone H3K27me3 Cellular Assay is a high-throughput screening (HTS) compatible TR-FRET assay for the interrogation of trimethylation at lysine 27 on histone H3 in a cellular format. Baculovirus-mediated gene delivery (BacMam) provides a convenient tool for the expression of GFP-Histone H3 fusion protein in your cell background of interest. Coupled with a terbium (Tb)-labeled site-specific antibody, the resulting cellular assay can be used to identify inhibitors of methyltransferases and demethylases acting on lysine 27 of histone H3

Features

• Use your cell background of choice with the portability of BacMam
• Identify more relevant inhibitors since the methyl transferases and demethylases are in their natural protein complexes
• Conserve precious cell samples with the miniaturizable homogenous assay format
• Improve data quality with the advantages of TR-FRE

Contents

• BacMam Histone H3 Reagent
• LanthaScreen™ Tb-anti-histone H3K27me3 Antibody
• 6X LanthaScreen™ Cellular Lysis Buffer
• Instrument Control Terbium TR-FRET kit