Invitrogen™ BacMam Histone H3K27me3 Cellular Assay Kit
On demand
Order number :
A14159
Invitrogen™ BacMam Histone H3K27me3 Cellular Assay Kit
The BacMam Histone H3K27me3 Cellular Assay is a high-throughput screening (HTS) compatible TR-FRET assay for the interrogation of trimethylation at lysine 27 on histone H3 in a cellular format. Baculovirus-mediated gene delivery (BacMam) provides a convenient tool for the expression of GFP-Histone H3 fusion protein in your cell background of interest. Coupled with a terbium (Tb)-labeled site-specific antibody, the resulting cellular assay can be used to identify inhibitors of methyltransferases and demethylases acting on lysine 27 of histone H3
Features
- Use your cell background of choice with the portability of BacMam
- Identify more relevant inhibitors since the methyl transferases and demethylases are in their natural protein complexes
- Conserve precious cell samples with the miniaturizable homogenous assay format
- Improve data quality with the advantages of TR-FRE
Contents
- BacMam Histone H3 Reagent
- LanthaScreen™ Tb-anti-histone H3K27me3 Antibody
- 6X LanthaScreen™ Cellular Lysis Buffer
- Instrument Control Terbium TR-FRET kit
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