Invitrogen™ BaculoDirect™ C-Term Transfection Kit
On demand
Order number :
12562039
Invitrogen™ BaculoDirect™ C-Term Transfection Kit
The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. The combination of Gateway™ Technology with baculovirus expression makes the BaculoDirect System the fastest and easiest method for production of recombinant baculovirus.
Features
- Strong polyhedrin promoter for high-level expression
- R-sites for efficient recombination with any attL-flanked Gateway entry vector
- TK gene for negative selection using ganciclovir
- C-terminal V5-His tag (BaculoDirect™ C-Term Expression Kit) or N-terminal V5-His tag (BaculoDirect™ N-Term Expression Kit) for detection with anti-V5 antibody and purification with nickel-chelating resin
- TEV protease cleavage site for removal of the V5-His tag following purification (BaculoDirect N-Term Expression Kit)
- LacZ gene, to ensure a pure baculovirus stock is generated, which is replaced by your gene of interest after LR recombinant reaction
Contents
- The BaculoDirect™ transfection kits include five 0.3 µg vials of BaculoDirect Linear DNA, 125 µL Cellfectin transfection reagent, and ganciclovir
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