Thermo Scientific™ pKT-Gaussia Luc Vector for Luciferase Assays

Thermo Scientific™ pKT-Gaussia Luc Vector for Luciferase Assays

The Thermo Scientific pTK-Gaussia Luc vector is a derivative of the pMCS-Gaussia Luc vector containing the Gaussia luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.

Features

• Weak (TK) constitutive promoters for co-transfection and normalization
• Naturally-secreting Gaussia luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin