Invitrogen™ BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit

Invitrogen™ BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit

The BLOCK-iT™ Lentiviral RNAi Zeo Gateway® Vector Kit contains the pLenti4/BLOCK-iT™-DEST expression vector which enables lentiviral delivery and genomic integration of DNA coding for shRNA. Once expressed, the shRNA is processed by cellular machinery and initiates target-specific RNAi. The pLenti4/BLOCK-iT™-DEST vector offers:

Gateway® Technology for efficient recombination of the RNAi cassette from the BLOCK-iT™ inducible pENTR™/H1/TO vector

Features

• All required components for efficient lentiviral packaging, delivery, and integration of the shRNA
• Zeocin™ selection marker for fast selection of clonal cell lines containing the RNAi cassette

Contents

• The BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit contains the pLenti4/BLOCK-iT™-DEST vector, pLenti4-GW/H1/TO-lamin shRNA control vector, and One Shot™ Stbl3™ Chemically Competent E.Coli. Store the pLenti4/BLOCK-iT™-DEST, pLenti6/TR vector, Blasticidin, ViraPower™ Lentiviral packaging mix, and the control vector at -20°C. Store the One Shot Stbl3™ Competent Cells 293 FT cell, and LR Clonase™ enzyme mix at -80°C. Store Lipofectamine™ 2000 at +4°C. All reagents are guaranteed stable for 6 months when properly stored.