Invitrogen™ BL21 Star™ (DE3)pLysS One Shot™ Chemically Competent E. coli

Invitrogen™ BL21 Star™ (DE3)pLysS One Shot™ Chemically Competent E. coli

One Shot™ BL21 Star™ (DE3) pLysS E. coli are chemically competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from high copy number, T7 promoter-based expression systems (e.g., pRSET T7 vectors). One Shot™ BL21 Star™ (DE3) pLysS Chemically Competent cells have a transformation efficiency of >1 x 108 cfu/ µg plasmid DNA.

Features

• High mRNA stability results in increased protein yield
• Ideal for use with high copy number, T7 promoter-based plasmids
• Low background expression in uninduced cells

Contents

• Both BL21 Star™(DE3) and BL21 Star™(DE3)pLysS Chemically Competent E. coli are provided in single-use 50 µl aliquots, with S.O.C. medium, and supercoiled control plasmid.