Invitrogen™ One Shot™ ccdB Survival™ 2 T1R Competent Cells

Invitrogen™ One Shot™ ccdB Survival™ 2 T1R Competent Cells

One Shot® ccdB Survival™ 2 T1R Chemically Competent Cells are suitable for the propagation of plasmids containing the ccdB gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and supercoiled entry vectors. Transformation efficiency is >1 × 109 transformants/µg plasmid DNA. The ccdB Survival™ 2 T1R E. coli strain is derived from the TOP10 strain

Features

• Resistance to the ccdB gene product
• Resistance to T1 and T5 phage (the tonA/fhuA phenotype)
• Supports high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype)
• Reduced nonspecific recombination of cloned DNA (the recA1 phenotype)

Contents

• ccdB Survival™2 T1R Competent Cells: 11 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml