Invitrogen™ One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli

Invitrogen™ One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli

One Shot® BL21 Star™ (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., Champion™ pET vectors). One Shot® BL21 Star™ (DE3) Chemically Competent cells are provided at a transformation efficiency of 1 x 108 cfu/µg plasmid DNA

Features

• Contain a genotype that promotes high mRNA stability and protein yield
• Optimized for use with low copy number, T7 promoter-based plasmids
• Provided in a single-tube, single-use format—allows all transformation steps to take place in the same tube, thereby helping to save time and prevent contamination

Contents

• One Shot™ BL21 Star™ (DE3) E.coli: 20 vials, 50 µl each (total of 1 ml)
• pUC19 DNA (10 pg/ul): 1 vial, 50 µl
• SOC Medium: 1 bottle, 6 ml