Invitrogen™ BLOCK-iT™ Inducible Pol II miR RNAi Expression Vector Kit with EmGFP

Invitrogen™ BLOCK-iT™ Inducible Pol II miR RNAi Expression Vector Kit with EmGFP

The BLOCK-iT™ Pol II miR RNAi Expression Vector Kits combine the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript. The BLOCK-iT™ Pol II miR RNAi Expression Vector Kits and the BLOCK-iT™ Lentiviral Pol II miR RNAi Expression Systems are designed to express artificial miRNAs which are engineered to have 100% homology to your target sequence and will result in target cleavage. Using Invitrogen’s award-winning BLOCK-iT™ RNAi Designer, over 70% of constructs produce more than 70% knockdown.

Features

• Strong expression from the CMV immediate early promoter, with the option to use tissue-specific or other regulated promoters via MultiSite Gateway® recombination.
• Compatibility with many of Invitrogen’s Gateway® destination (DEST) vectors for gene expression.

Contents

• The BLOCK-iTTM Inducible PolII miR RNAi Expression Vector Kit w/EmGFP contains a cloning box with linearized pcDNA™6.2-GW/EmGFP-miR, 10X annealing buffer, T4 DNA Ligase, 5X DNA Ligation buffer, lacZ control oligo, lacZ control plasmid, negative control plasmid, DNase/RNase-free water, and forward and reverse sequencing primers. This kit also contains the pT-REx-DEST30 Gateway™ Vector kit (includes pT-REx-DEST30 and pT-REx/GW-30/lacZ vectors), and the T-RExTM Regulatory Module (includes pcDNA™6/TR and Tetracycline). The Gateway™ LR and BP Clonase™ kits, blasticidin, and pDONRTM221 are included. Store the vectors, buffers, control oligo and plasmids, antibiotics, clonase kits, water, and sequencing primers at -20°C. The One Shot™ box contains transformation reagents including twenty-one 50-µl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and a pUC19 supercoiled control plasmid. Store these transformation reagents -80°C.