Invitrogen™ Vivid Colors™ pcDNA™6.2/EmGFP-Bsd/V5-DEST Vector

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Brand origin : United States

Expiry time : On demand

Delivery time : 1 Week/s

Delivery cost : On demand

Unit size : 6 µg

Order number : V36620

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Invitrogen™ Vivid Colors™ pcDNA™6.2/EmGFP-Bsd/V5-DEST Vector

The Vivid Colors™ pcDNA™ 6.2/EmGFP-Bsd/V5-DEST vector allows you to rapidly clone your gene using Gateway® technology and simultaneously express it with Emerald Green Fluorescent Protein (EmGFP) for easy identification of transfected cells. The EmGFP is derived from well-characterized fluorescent proteins of the jellyfish Aequorea victoria (1, 2) and has been humanized for optimal mammalian expression (3). If cloned with the TAG (amber) codon, your gene will be expressed in its native configuration and fully compatible with Tag-On-Demand" technology. If cloned without a stop codon, a V5-epitope tag will be fused to the carboxy-terminal end of your protein of interest. In either case, expression of EmGFP provides a bright fluorescent marker to easily identify cells co-expressing your protein. This marker allows you to focus your functional analysis on specific cells, or to sort and enrich for transfected cells in a derived population.

Features

PGK promoter for high-level expression of EmGFP fused to the Blasticidin (bsd)-resistance marker
CMV promoter for high-level expression of your protein
V5-epitope tag option to fuse to the C-terminal end of your protein, if desired

pcDNA™6.2/EmGFP-Bsd/V5-DEST: 6 μg
pcDNA™6.2/EmGFP-Bsd/V5-GW/CAT: 10 μg

Cloning Method

Gateway™

Constitutive or Inducible System

Constitutive

Promoter

CMV

Protein Tag

V5 Epitope Tag

Reporter Gene

GFP (EmGFP)

Selection Agent (Eukaryotic)

Blasticidin