Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ MAX Efficiency DH5α-T1R E. coli, 50 Reactions
On demand
Order number :
K282040
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ MAX Efficiency DH5α-T1R E. coli, 50 Reactions
Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR™-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase.
Features
- ccdB gene for positive selection
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts
- Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli
- SP6 promoter/primer site for in vitro RNA transcription and sequencing
- M13 forward and reverse primer sites for sequencing or PCR screening
Contents
Box 1:
- Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
- Salt solution
- dNTPs
- Control template
- M13 forward and reverse primers
- Sterile water
Box 2:
- One Shot™ Chemically Competent or Electrocomp™ E. coli
- S.O.C. medium
- Supercoiled pUC19 control plasmid
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