Invitrogen™ CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent

Invitrogen™ CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent

CellEvent™ Caspase-3/7 Green Ready Flow™ Reagent enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The product includes 2 reagents: the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent and SYTOX™ AADvanced™ Dead Cell Stain.
CellEvent Caspase-3/7 Green Ready Flow Reagent is a cell-permeant reagent that detects caspase 3/7 activity using flow cytometry. Add 2 drops from the dropper bottle directly into 1 x 10⁶ cells, incubate, and analyze.
CellEvent Caspase-3/7 Green Ready Flow Reagent consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorescent signal having absorption/emission maxima of ∼502/530 nm. When used together with the SYTOX AADvanced Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.

Features

• Stable at room temperature.
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette.
• Exceptionally bright green fluorescence upon binding to nucleic acids.
• Rapid staining of apoptotic cells without wash steps.

Contents

• 4 dropper bottles.