Invitrogen™ Cells-to-cDNA™ II Kit, 40 Reactions

Invitrogen™ Cells-to-cDNA™ II Kit, 40 Reactions

The Ambion® Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr. No RNA isolation is required. This kit contains sufficient reagents for 100 reactions and is ideal for those who want to perform reverse transcription reactions on small numbers of cells, numerous cell samples, or for scientists who are unfamiliar with RNA isolation.

Features

• No RNA purification required
• From cells in culture to cDNA in less than 2 hr
• Detect rare messages in as few as 1 cell
• Ideal for labs not equipped for RNA isolation

Contents

•  PBS (pH 7.4): 1X40 mL
• Cell Lysis II Buffer: 4 mL
• DNase 1 (2 U/μL): 80 μL
• RT Buffer: 10X200 μL
• M-MLV Reverse Transcriptase: 40 μL
• RNase Inhibitor (10 U/μL): 40 μL
• dNTP Mix (2.5 mM each dNTP): 160 μL
• Random Decamers (50 μM): 80 μL
• Oligo(dT)18 Primers (50 μM): 80 μL
• Armored RNA® Contro: 50 μL
• Armored RNA® Primer Pair (10 μM e): 80 μL
• Endogenous Primer Pair (5 μM ea): 80 μL
• Nuclease-free Water: 1.75 mL