Invitrogen™ Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit

Invitrogen™ Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit

The Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 594 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 594 azide leads to a chemoselective ligation or “click' reaction between the green fluorescent azide and the alkyne, allowing the modified proteins to be detected by imaged-based analysis.

For use with equipment: Fluorescence Microscope, Fluorescent Imager

Features  

• Non-radioactive alternative—an alternative to the traditional 35S-methionine
• Visualize bulk protein dynamics—fluorescent tagging of proteins allows their localization to be determined, including aggregation
• Specificity—selective, specific reaction between label and detection tags
• Stability —product contains an irreversible, covalent bond
• Multiplex-enabled—use in conjuction with Click®-iT AHA (azide amino acid and alkyne dye) to detect spatial and temporal differences
• Applicability to biological samples—easy detection; high sensitivity and low background, regardless of complexity