Invitrogen™ Alexa Fluor™ 680 Antibody Labeling Kit

Invitrogen™ Alexa Fluor™ 680 Antibody Labeling Kit

Molecular Probes® Alexa Fluor® antibody labeling kits provide a convenient means to label small amounts of antibodies with Alexa Fluor® dyes (choice of 10 colors). This kit is optimized for labeling 100 μg of antibody per reaction with infrared fluorescent Alexa Fluor® 680. Small amounts of other proteins (>40 kDa) can also be labeled with this kit.

The Alexa Fluor® 680 Antibody Labeling Kits contains everything you need to perform five separate labeling reactions as well as to purify the resulting conjugates. The conjugates are ideal for multiple applications, including small animal in vivo imaging, flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, indirect FISH, and more.

With an excitation and emission maximum of 684/707 nm, Alexa Fluor® 680 can be efficiently excited and detected in most standard small animal in vivo imaging and fluorescent western blot imaging systems.

Features 

•  With an excitation and emission maximum of 530/554 nm, Alexa Fluor® 532 can be efficiently excited using a 532 nm Nd:YAG laser line and detected under standard Rhodamine 6G filters 
• Labeled proteins typically ready to use in 90 min (∼15 min hands-on time)
• Optimized for small-scale labeling of any protein >40 kDa
• Conjugates purified using convenient spin filters
• Stabilizing proteins must be removed from the sample before labeling
• Includes detailed instructions for determining degree of labeling (DOL)

Contents 

• Alexa™ 680 Antibody Labeling Kit comes with 5 vials of reactive dye, sodium bicarbonate, purification resin, spin columns, and collections tubes.