Invitrogen™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry, FITC, Propidium Iodide, 50 Assays

Invitrogen™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry, FITC, Propidium Iodide, 50 Assays

The Dead Cell Apoptosis Kit with Annexin V FITC and PI detects PS externalization in apoptotic cells using recombinant annexin V conjugated to green-fluorescent FITC dye and dead cells using PI. Necrotic cells that stain with PI show red fluorescence. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.

Features

• Superior brightness, Unlike other Annexin V kits that have lower protein concentrations or purification levels, the Annexin V Alexa Fluor 488 conjugate is optimized for flow cytometry to provide the largest separation between apoptotic and live cells. The Alexa Fluor 488 dye is a superior green-fluorescent dye with a spectrum similar to fluorescein (FITC).
• High binding efficiency, Annexin V conjugates are made from a highly purified cys-annexin, resulting in higher binding efficiency and extremely accurate characterization of the apoptotic process.
• Multi-parametric, Many publications require a minimum of two different ways to identify apoptotic cells. This multi-parametric kit detects phosphatidyl serine (PS) on the cytoplasmic surface of the cell membrane, while membrane integrity is determined via propidium iodide.

Contents

• 1 vial of annexin V, FITC conjugate (250 µL ).
• 1 vial of propidium iodide (PI, 100 µL).
• 1 bottle of annexin binding buffer (5X solution, 15 mL).